Dependency rights on a order

Hi, all!

Could anyone clarify, do user's access rights depend on the order in which organizational roles are added on the detail of the user's form? I mean is it important if I add organizational roles for one user in the order

Role1

Role2

Role 3

and for another user in the order 

Role 2

Role 1

Role 3

And give them the same functional role (only one)?

I'm asking because now those two users can see different amount of the records of the same object and the only difference in their rights is that order.